Ultrapure, high performance dNTPs

Free from: Bacterial and Human DNA PCR Inhibitors DNases, RNases Nicking Enzymes Proteases

Individual dNTPs

Individual solutions of ultrapure deoxyribonucleotide (dNTPs) for use in PCR applications.
Solutions with custom dNTP concentrations, as well as custom countercations are available on request.
Our dNTP solutions are available in quantities from 50 mL to 1000 L. Packaging sizes of 0.25 mL to 200 mL.
Samples are available on request.

Features

  • Ultrapure (>99.0% purity)
  • Exceptional stability
  • Free of human and E. coli DNA
  • Free of DNases, RNases and proteases

Applications

  • Long-range PCR
  • cDNA synthesis and RT-PCR
  • Real-time PCR
  • Standard PCR
  • High-fidelity PCR
  • 2'-Deoxyadenosine-5'-triphosphate, Sodium salt
  • 2'-Deoxycytidine-5'-triphosphate, Sodium salt
  • 2'-Deoxyguanosine-5'-triphosphate, Sodium salt
  • 2'-Deoxythymidine-5'-triphosphate, Sodium salt
  • 2'-Deoxyuridine-5'-triphosphate, Sodium salt

Premixed dNTPs

Premixed solutions of ultrapure deoxyribonucleotide (dNTPs) for ease of use in PCR applications. Standard mixes contain equimolar concentrations of dATP, dCTP, dGTP, and dTTP.
Custom premixes with different concentrations, or including dUTP, are available on request.
Our dNTP mixes are available in quantities from 50 mL to 1000 L. Packaging sizes of 0.25 mL to 200 mL.
Samples are available on request.

Features

  • Ultrapure (>99.0% purity)
  • Exceptional stability
  • Free of human and E. coli DNA
  • Free of DNases, RNases and proteases

Applications

  • Long-range PCR
  • cDNA synthesis and RT-PCR
  • Real-time PCR
  • Standard PCR
  • High-fidelity PCR
  • 2'-Deoxyadenosine-5'-triphosphate, Sodium salt,
    2'-Deoxycytidine-5'-triphosphate, Sodium salt,
    2'-Deoxyguanosine-5'-triphosphate, Sodium salt,
    2'-Deoxythymidine-5'-triphosphate, Sodium salt

Made to Measure Reagents

As an original manufacturer, we can provide tailor-made products catering to your specific requirements. This includes custom formulations, custom concentrations, as well as filling and custom labelling for distributors who we supply to as an OEM.

OEM

Whether you are a reagent manufacturer looking to purchase raw materials, a re-seller looking for an Original Equipment Manufacturer (OEM), or a diagnostic and research laboratory needing PCR reagents, we can provide the high quality materials that you need.

ISO 9001 Certified Quality Management System

To ensure that we maintain a consistent level of quality, we developed and implemented a quality and environmental management system certified under the international standards DIN EN ISO 9001:2015 and DIN EN ISO 14001:2015.

Production Technology

LAROVA is one of only a few primary manufacturers of dNTPs for PCR. Our high quality starts with our production technology. Many problematic impurities, such as pyrophosphate and modified nucleotides, are by-products from chemical synthesis.
These impurities can severely impact PCR performance. That’s why we synthesise all our dNTPs enzymatically, meaning many common impurities are never even present in our solutions. Any remaining impurities are removed with several state-of-theart purification procedures.
For dATP, dCTP, dGTP, and dUTP, we start with the respective ribonucleotide, and use the highly specific Ribonucleotide Reductase enzyme (Scheme 1). While for dTTP, we use enzymes to sequentially phosphorylate thymidine.



Scheme 1. The bacterial enzyme ribonucleotide reductase selectively reduces the 2’-OH-group of the selected ribonucleotide (rNTP) to give the corresponding deoxyribonucleotide (dNTP). Our enzymatic syntheses are performed in this manner on a multi-kilogram scale.