The available portfolio of our polymerases allows choosing the most appropriate enzyme for a particular application.
Enzyme | Efficiency /Yield | Specifity | Fidelity /Error rate [1] | Application |
---|---|---|---|---|
Taq Polymerase | ++ | ++ | 10-5 |
|
Hot Start Polymerase | ++ | +++ | 10-5 |
|
High Fidelity Polymerase | +++ | ++ | 2x10-6 |
|
High Fidelity Hot Start Polymerase | +++ | +++ | 2x10-6 |
|
Pfu-X Polymerase | +++ | +++ | 2x10-7 |
|
[1] The error rate of a polymerase is calculated as number of mutations per number of base pairs per DNA doublings (PCR cycles).
ER = MF / (bp · d)
ER: Error rate
MF: number of mutations (mutation frequency)
bp: number of base pairs (fragment length)
d: DNA doublings (number of PCR cycles)
LAROVA Polymerase references
Singh et al. (2012) Cloning, expression and characterization of a metagenome derived thermoactive/thermostable pectinase. Molecular Biology Reports 39:8353.
Yehuda et al. (2012) Isothiocyanates inhibit psoriasis-related proinflammatory factors in human skin. Inflamm. Res. 61:735.
Aggarwal et al. (2011) Agrobacterium tumefaciens mediated genetic transformation of selected elite clone(s) of Eucalyptus tereticornis. Acta Physiol. Plant 33:1603.
Babu et al. (2011) Diversity of Arbuscular Mycorrhizal Fungi Associated with Plants Growing in Fly Ash Pond and Their Potential Role in Ecological Restoration. Curr. Microbiol. 63:273.
General references
Kim et al. (2008) Crystal structure of Pfu, the high fidelity DNA polymerase from Pyrococcus furiosus. Int. J. Biol. Macromol. 42:356.
Birch et al. (1996) Simplified hot start PCR. Nature 381:445.
Cline et al. (1996) PCR Fidelity of Pfu DNA Polymerase and Other Thermostable DNA Polymerases. Nucleic Acids Res. 24-18:3546.
Myers et al. (1991) Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase. Biochemistry 30 (31):7661.
Lundberg et al. (1991) High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus. Gene. 108:1.