The Nucleotide Company
 

DNA Polymerases

The available portfolio of our polymerases allows choosing the most appropriate enzyme for a particular application.

Enzyme Efficiency /Yield Specifity Fidelity /Error rate [1] Application
Taq Polymerase++++10-5
  • Standard PCR / optimized for minimal by-product formation
  • Routine and plate based PCR, automated pipetting
  • Taq Polymerase /high yield++++10-5
  • Standard PCR / optimized for high efficiency in a broad range of reaction conditions
  • Incorporation of modified nucleotides
  • Hot Start Polymerase+++++10-5
  • High specificity PCR / high sensitivity PCR
  • Plate based PCR and automated pipetting
  • High Fidelity Polymerase+++++2x10-6
  • High fidelity PCR / long range PCR (> 30 kb)
  • Amplification of GC-rich and other difficult templates
  • High Fidelity Hot Start Polymerase++++++2x10-6
  • High fidelity PCR with highest specificity and sensitivity
  • Long range PCR, amplification of difficult templates and of small template amounts
  • Pfu-X Polymerase ++++++2x10-7
  • Amplification with highest fidelity
  • High speed amplification of difficult and long templates
  • [1] The error rate of a polymerase is calculated as number of mutations per number of base pairs per DNA doublings (PCR cycles).

      ER = MF / (bp · d)
      ER: Error rate
      MF: number of mutations (mutation frequency)
      bp: number of base pairs (fragment length)
      d: DNA doublings (number of PCR cycles)

    Selected references

    LAROVA Polymerase references

    Singh et al. (2012) Cloning, expression and characterization of a metagenome derived thermoactive/thermostable pectinase. Molecular Biology Reports 39:8353.

     Download PDF

     

    Yehuda et al. (2012) Isothiocyanates inhibit psoriasis-related proinflammatory factors in human skin. Inflamm. Res. 61:735.

     Download PDF

     

    Aggarwal et al. (2011) Agrobacterium tumefaciens mediated genetic transformation of selected elite clone(s) of Eucalyptus tereticornis. Acta Physiol. Plant 33:1603.

     Download PDF

     

    Babu et al. (2011) Diversity of Arbuscular Mycorrhizal Fungi Associated with Plants Growing in Fly Ash Pond and Their Potential Role in Ecological Restoration. Curr. Microbiol. 63:273.

     Download PDF

     

    General references

    Kim et al. (2008) Crystal structure of Pfu, the high fidelity DNA polymerase from Pyrococcus furiosus. Int. J. Biol. Macromol. 42:356.

     Download PDF

     

    Birch et al. (1996) Simplified hot start PCR. Nature 381:445.

     Download PDF

     

    Cline et al. (1996) PCR Fidelity of Pfu DNA Polymerase and Other Thermostable DNA Polymerases. Nucleic Acids Res. 24-18:3546.

     Download PDF

     

    Myers et al. (1991) Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase. Biochemistry 30 (31):7661.

     Download PDF

     

    Lundberg et al. (1991) High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus. Gene. 108:1.

     Download PDF