DNA Polymerases – Applications

The available portfolio of our polymerases allows choosing the most appropriate enzyme for a particular application.

Enzyme	Efficiency /Yield	Specifity	Fidelity /Error rate ^[1]	Application
Taq Polymerase	++	++	10^-5	Standard PCR / optimized for minimal by-product formation Routine and plate based PCR, automated pipetting
Hot Start Polymerase	++	+++	10^-5	High specificity PCR / high sensitivity PCR Plate based PCR and automated pipetting
High Fidelity Polymerase	+++	++	2x10^-6	High fidelity PCR / long range PCR (> 30 kb) Amplification of GC-rich and other difficult templates
High Fidelity Hot Start Polymerase	+++	+++	2x10^-6	High fidelity PCR with highest specificity and sensitivity Long range PCR, amplification of difficult templates and of small template amounts
Pfu-X Polymerase	+++	+++	2x10^-7	Amplification with highest fidelity High speed amplification of difficult and long templates

^[1] The error rate of a polymerase is calculated as number of mutations per number of base pairs per DNA doublings (PCR cycles).

ER = MF / (bp · d)
ER: Error rate
MF: number of mutations (mutation frequency)
bp: number of base pairs (fragment length)
d: DNA doublings (number of PCR cycles)

Selected references

LAROVA Polymerase references

Singh et al. (2012) Cloning, expression and characterization of a metagenome derived thermoactive/thermostable pectinase. Molecular Biology Reports 39:8353.

Download PDF

Yehuda et al. (2012) Isothiocyanates inhibit psoriasis-related proinflammatory factors in human skin. Inflamm. Res. 61:735.

Download PDF

Aggarwal et al. (2011) Agrobacterium tumefaciens mediated genetic transformation of selected elite clone(s) of Eucalyptus tereticornis. Acta Physiol. Plant 33:1603.

Download PDF

Babu et al. (2011) Diversity of Arbuscular Mycorrhizal Fungi Associated with Plants Growing in Fly Ash Pond and Their Potential Role in Ecological Restoration. Curr. Microbiol. 63:273.

Download PDF

General references

Kim et al. (2008) Crystal structure of Pfu, the high fidelity DNA polymerase from Pyrococcus furiosus. Int. J. Biol. Macromol. 42:356.

Download PDF

Birch et al. (1996) Simplified hot start PCR. Nature 381:445.

Download PDF

Cline et al. (1996) PCR Fidelity of Pfu DNA Polymerase and Other Thermostable DNA Polymerases. Nucleic Acids Res. 24-18:3546.

Download PDF

Myers et al. (1991) Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase. Biochemistry 30 (31):7661.

Download PDF

Lundberg et al. (1991) High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus. Gene. 108:1.

Download PDF