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Blue Gel Loading Buffer
Cat. No. Amount Price (EUR) Downloads
PCR-254S-bl 5x 1 ml 25,00  
PCR-254L-bl 5x 5 ml 100,00  


Store at 2-8°C

6x concentration

Blue Loading Buffer is formulated to facilitate loading of DNA containing samples into the wells of agarose and polyacrylamide gels. The buffer contains Bromophenol Blue and Xylene Cyanol FF as tracking dyes and indicator for the DNA fragment migration. In addition, it contains glycerol to add density and EDTA to inhibit nuclease activities.
The buffer is recommended for loading of DNA fragments larger than 100 bp.
 
 
Orange Gel Loading Buffer
Cat. No. Amount Price (EUR) Downloads
PCR-254S-or 1 ml 25,00  
PCR-254L-or 5x 5 ml 100,00  


Store at 2-8°C

6x concentration

Orange Loading Buffer is formulated to facilitate loading of DNA containing samples into the wells of agarose and polyacrylamide gels. The buffer contains Orange G as tracking dye and indicator for the DNA fragment migration. In addition, it contains glycerol to add density and EDTA to inhibit nuclease activities.
The buffer is recommended for loading of small DNA fragments up to 200 bp.
 
 
PCR Control Kit
Cat. No. Amount Price (EUR) Downloads
PCR-253 500 reactions 50,00  


store at -20°C

ß-actin forward Primer
ß-actin reverse Primer
Human genomic DNA (male)

PCR Control Kit is designed for quality and benchmark tests, as positive control or internal lab standard for Taq DNA polymerases. The kit contains a forward / reverse primer-pair designed to amplify a 295 bp long fragment of the β-actin gene from human genomic DNA.
 
 
PCR Additives Kit
Cat. No. Amount Price (EUR) Downloads
PCR-252 500 reactions 40,00  


Store at 2-8°C or -20°C

Taq-stabilizer
GC-enhancer

PCR Additives Kit contains a stabilizer and enhancer bundle for the amplification of difficult templates such as GC-rich or extremly long fragments.
Taq-stabilizer
5-10% Taq-stabilizer in the PCR reaction is recommended to improve amplification efficiency and specificity. It stabilizes Taq polymerase and reduces secondary DNA structures. Note that higher concentrations may give conflicting results in multiplex reactions.
GC-enhancer
15-30% GC-enhancer in the PCR is recommended for amplifying GC-rich templates. It reduces the melting temperature of DNA. Thus, primer annealing temperature should be reduced by 1-2°C.
 
 
 
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