Taq Polymerase
| Cat. No. | Amount | Price (EUR) | Downloads |
| PCR-202S | 200 units | 35,00 |  |
| PCR-202L | 1000 units | 140,00 | |
Store at -20°C, avoid frequent thawing and freezing
Taq Polymerase (5 units/µl)
10x Taq reaction buffer complete
10x Taq reaction buffer without MgCl2
MgCl2 stock solution
Taq Polymerase is recommended for use in routine PCR reactions. The buffer system is optimized for high specificity and guarantees minimal by-product formation. It is particularly suitable for plate based PCR and automated pipetting where a detergent free buffer system is required.
The enzyme replicates DNA at 72°C. It catalyzes the polymerization of nucleotides into duplex DNA in 5'→3' direction in the presence of magnesium. It also possesses a 5'→3' polymerization-dependent exo-nuclease replacement activity but lacks a 3'→5' exonuclease activity.
Taq Polymerase / high yield
| Cat. No. | Amount | Price (EUR) | Downloads |
| PCR-201S | 200 units | 35,00 | |
| PCR-201L | 1000 units | 140,00 | |
Store at -20°C, avoid frequent thawing and freezing
Taq Polymerase (5 units/µl)
10x High yield buffer complete
10x High yield buffer without MgCl2
MgCl2 stock solution
Taq Polymerase / high yield is recommended for use in routine PCR reactions. The buffer system is optimized for high efficiency and gives superior amplification results in a broad range of reaction conditions with most primer-template pairs. Note that the ammonium based buffer system contains detergent and is not recommended for plate based PCR and automated pipetting.
The enzyme replicates DNA at 72°C. It catalyzes the polymerization of nucleotides into duplex DNA in 5'→3' direction in the presence of magnesium. It also possesses a 5'→3' polymerization-dependent exo-nuclease replacement activity but lacks a 3'→5' exonuclease activity.
Hot Start Polymerase
| Cat. No. | Amount | Price (EUR) | Downloads |
| PCR-203S | 200 units | 70,00 | |
| PCR-203L | 1000 units | 280,00 | |
Store at -20°C, avoid frequent thawing and freezing
Hot Start Polymerase (5 units/µl)
10x Hot start buffer complete
10x Hot start buffer without MgCl2
MgCl2 stock solution
Hot Start Polymerase provides improved specificity and sensitivity when amplifying low-copy-number targets in complex backgrounds or when prolonged room-temperature set up is required. The polymerase activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup.
The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5'→3' direction in the presence of magnesium. It also possesses a 5'→3' polymerization-dependent exonuclease replacement activity but lacks a 3'→5' exonuclease activity.
Activation step
The Hot Start Polymerase requires no prolonged heating or denaturing step. The polymerase inhibiting ligand is quickly released at the increased temperature of thermal cycling.
High Fidelity Polymerase
| Cat. No. | Amount | Price (EUR) | Downloads |
| PCR-204S | 100 units | 48,00 | |
| PCR-204L | 500 units | 192,00 | |
Store at -20°C, avoid frequent thawing and freezing
High Fidelity Polymerase (2.5 units/µl)
10x High fidelity buffer
High Fidelity Polymerase is based on a blend of Taq DNA polymerase and a proofreading enzyme specially designed for highly accurate and efficient amplification. It shows excellent results with extremely long (up to 30 kb), GC-rich or other difficult templates.
The enzyme blend includes a highly processive 5'→3' DNA polymerase and possesses a 5'→3' polymerization-dependent exonuclease replacement activity. Its inherent 3'→5' exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase.
The enzyme is highly purified and free of bacterial DNA.
Pfu-X Polymerase
| Cat. No. | Amount | Price (EUR) | Downloads |
| PCR-207S | 100 units | 60,00 | |
| PCR-207L | 500 units | 240,00 | |
Store at -20°C, avoid frequent thawing and freezing
Pfu-X Polymerase (2.5 units/µl)
10x Pfu-X buffer <3kb
10x Pfu-X buffer >3kb
Pfu-X Polymerase is the ideal choice for applications where the efficient amplification of DNA with highest fidelity is required.
The enzyme is a genetically engineered Pfu DNA polymerase, but showing a 2-fold higher accuracy and an increased processivity, resulting in shorter elongation times.
The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5'→3' direction and possesses a 5'→3' polymerization-dependent exonuclease replacement activity. Its inherent 3'→5' exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase. Pfu-X Polymerase-generated PCR fragments are blunt-ended.
The enzyme is highly purified and free of bacterial DNA.