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Properties of Fluorescent Dyes

  



Dye/
Fluorescent Color
MW
(free acid)
λabsλemεmaxIsomersNotes/Advantages

[g/mol][nm][nm][cm-1*M-1]

DY-776835771801120,000puresoluble in water, negatively charged, spectral similar to IRD 800
DY-751815751779220,000pure
ATTO 740568740764120,000pureAlternative dye for LICOR applications
ATTO 725516725752120,000pureAlternative dye for LICOR applications
ATTO 700575700719120,000pureSubstitute for Cy5.5
ATTO 680631680700125,000pureSubstitute for Cy5.5
ATTO 655634663684125,000pureSubstitute for Cy5, but better since no triplet behaviour
OYSTER-656737656674200,000pureHigher photostability compared to Cy5
ATTO 647N746644669150,000twoSubstitute for Alexa Fluor 647 and Cy5, base stable
ATTO 633652629657130,000pureEmission maximum of He-Ne laser, base stable, less hydrophilic
ATTO Rho14784625646130,000pure
ATTO 620612619643120,000pureSubstitute for LightCycler Red 640, base stable
DY-480XL51550063050,000pureLarge Stokes Shift of 130 nm
ATTO 5941137601627120,000pureSubstitute for Alexa-594, more hydrophilic than ATTO 590
ATTO Rho13646600625125,000pure
ATTO 590691594624120,000twoSubstitute for Texas Red / Alexa-594
ATTO Rho101590586610120,000pure
Texas Red72558860980,000pure
ATTO Thio12503579609110,000pure
ATTO Rho12650576601120,000pure
6-ROX53557560082,000pure
ATTO Rho11567571595120,000pure
ATTO 565611563592120,000twoSubstitute for Cy3 / Alexa Fluor 546 / Bodipy 558/568, better photostability
ATTO 550694554576120,000three
(60:22:17)
Substitute for Cy3 / Alexa Fluor 546 / Bodipy 558/568, better photostability
5/6-TAMRA43054557590,000two
Cy3632550570150,000pure
ATTO Rho6G514532560115,000pure
DY-485XL50348556050,000pureLarge Stokes Shift of 75 nm
ATTO 532765532553115,000pureSubstitute for Alexa Fluor 532 / Bodipy 530/550
6-JOE50452054875,000pureSubstitute for VIC / HEX
ATTO 49545249552780,000pure
ATTO 48880450152390,000pureSubstitute for FITC / Alexa Fluor 488 / Cy2, identical to Alexa, much more photostable than Cy2 or FITC
6-FAM37649251783,000pure
5-FAM37649251783,000pure
ATTO 46539645350875,000pureSubstitute for Cyan 500
ATTO 42540143648445,000pure
ATTO 39034339047924,000pure
MANT1513354402,700pure


 
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